Tsukamurella tyrosinosolvens – An unusual case report of bacteremic pneumonia after lung transplantation
Armelle Ménard (1,2,3), Sébastien Degrange (1), Olivia Peuchant (1), Thi DT Nguyen (1), Claire Dromer (4) and Jeanne Maugein (1)*
Armelle Ménard: email@example.com
Sébastien Degrange: Sebastien.Degrange@bioffice.fr
Olivia Peuchant: Olivia.Peuchant@u-bordeaux2.fr
Thi Diem Tien Nguyen: firstname.lastname@example.org
Claire Dromer: Claire.email@example.com
* Corresponding author: Jeanne Maugein firstname.lastname@example.org
(1) Laboratoire de Bactériologie – C.H.U. de Bordeaux, Hôpital Pellegrin et Hôpital du Haut-Lévêque – Place Amélie Raba-Léon – 33076 Bordeaux cedex, France
(2) Laboratoire de Bactériologie Université Victor Segalen Bordeaux 2, EA 3667, F33076 Bordeaux, France
(3) INSERM U853, F33076 Bordeaux, France
(4) Service de chirurgie thoracique, Hôpital du Haut-Lévêque, F33604 Pessac, France
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Annals of Clinical Microbiology and Antimicrobials 2009, 8:30 doi:10.1186/1476-0711-8-30
The electronic version of this article is the complete one and can be found online at: http://www.ann-clinmicrob.com/content/8/1/30
Received: 25 May 2009; Accepted: 12 November 2009; Published: 12 November 2009
© 2009 Ménard et al; licensee BioMed Central Ltd.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Lung transplant recipients have an increased risk for actinomycetales infection secondary to immunosuppressive regimen.
A case of pulmonary infection with bacteremia due to Tsukamurella tyrosinosolvens in a 54-year old man who underwent a double lung transplantation four years previously is presented.
The identification by conventional biochemical assays was unsuccessful and hsp gene sequencing was used to identify Tsukamurella tyrosinosolvens.
Bacteria of the genus Tsukamurella are included in the order Actinomycetales, characterized by mycolic acids. Phylogenetic analyses have demonstrated that the genus Tsukamurella is related to the genera Nocardia, Rhodococcus, Dietzia, Gordonia, Streptomyces, Corynebacterium and Mycobacterium [1,2]. The name of Tsukamurella comes from Tsukamura, a microbiologist who in 1971 described the first strain of Gordona aurantiaca, isolated from sputum of a patient who was affected by a chronic lung pathology . Some Tsukamurella species are commensal bacteria found in the environment, both at ground level and in water, and are opportunistic bacteria in patients with chronic pulmonary infection or in immunocompromized patients [4,5]. To date, T. tyrosinosolvens has been isolated only from human specimens and has always been associated with clinical disease .
In this report, we describe the first isolation of T. tyrosinosolvens from sputum and blood samples of a lung transplant patient.
A 54-year old man was hospitalized in July 2008 for a pulmonary infection. His medical history indicated 1) a lung transplant in November 2004 for emphysema linked to an alpha-1-antitrypsin deficiency, 2) chronic respiratory insufficiency, and 3) hypertension. In addition, several infectious episodes were noted in 2005 and 2006 following a fracture of the left femoral neck in May 2005 with severe osteoporosis and a fracture of the right foot in June 2006.
Upon admission, the patient described a chronic cough with abundant expectorations during the previous ten days. No fever was noted. The bronchial endoscopy showed of the presence of abundant secretions in the right bronchial tree. The mucous was notably inflammatory. The bronchial biopsies revealed acute bronchitis signs. A broncho-alveolar fluid sample was sent to the bacteriology laboratory and the analysis showed 4 × 107 cells/mL with 93% polymorphs. The presence of Gram-positive bacilli was noted, but Ziehl-Neelsen staining was negative.
A few hours post- endoscopy, a fever appeared (temperature up to 40°C) and three series of aerobic and anaerobic blood cultures were done. Blood culture showed only Gram-positive bacilli from the three aerobic bottles after 5 days which were identical to those identified in the broncho-alveolar fluid grown at the same time.
A therapy associating imipenem and tobramycin led to a significant clinical improvement, with a reduction in sputum volume, cough and apyrexa within 3 days. A cardiac transesophagal echography was performed to rule out endocarditis. The evolution was favorable with an important decrease in the inflammatory syndrome, the C-reactive proteins decreased from 103 to 9.3 mg/L.
With regard to the phenotypic identification, the isolate grew aerobically on blood agar at 37°C forming colonies with an unusual morphology. The colonies were rough and yellowish, similar to fungal growth. Phenotypic identification of Gram-positive organisms was performed employing the API Corynebacterium System (bioMérieux, Marcy l’Eoile, France) and other biochemical tests, including detection of arylsulfatase, lysozyme growth, and hydrolysis of casein, xanthine and tyrosine. They were all interpreted using conventional methods. The isolate was further examined using the GenoType Mycobacteria Molecular Genetic Assay (Hain Lifescience, Nehren, Germany); although both conjugate and universal controls were positive, the isolate failed to hybridize with the 13 bacterial species targetted by the kit. Antimicrobial susceptibility testing was carried out using a diffusion method. The strain was resistant to penicillin G, ampicillin, oxacillin, cefalotin, ceftriaxone, piperacillin/tazobactam, erythromycin, spiramycin and pristinamycin but was sensitive to imipenem, ertapenem, doxycycline, tigecycline, cotrimoxazol, rifampicin, vancomycin and linezolid. As identification based on this technology and the biochemical profile was unsuccessful, we turned to genotyping.
Concerning the genotypic identification, a 16S rRNA gene sequencing was first performed with the universal forward 27F (5′-AGAGTTTGATCMTGGCTCAG-3′) and reverse 1492R (5′-TACGGYTACCTTGTTACGACTT-3′) primers [7,8] generating amplicons of 1485 bp. PCR was performed with the Taq DNA polymerase (Promega, Charbonnières-les-Bains, France). Amplification parameters consisted of one cycle at 95°C for 5 min, followed by 35 cycles at 95°C for 30 s, 58°C for 30 s, and 72°C for 2 min, and finally one cycle at 72°C for 5 min. The products of PCR amplification were purified using MicroSpin S-400 HR columns (Amersham Pharmacia Biotech Inc., Uppsala, Sweden) and sequencing of these products was achieved on both strands using the initial set of PCR primers, and internal primers F1-16S-Seq-Univ (5′-GCTAACTCCGTGCCAGCAG-3′) and R1-16S-Seq-Univ (5′-TTGCGGGACTTAACCCAAC-3′), using an Applied Biosystems 3130 xl Genetic Analyzer (Applied Biosystems, Foster City, CA, USA) with the fluorescent Big Dye terminator V1.1 Cycle Sequencing Kit (Applied Biosystems), according to the manufacturer’s instructions. Amplified primer-less sequences were compared to the GenBank database with the nucleotide Blast program at the National Center for Biotechnology Information Computer server  http://blast.ncbi.nlm.nih.gov/Blast.cgi webcite. The sequence showed 99% identity in a 1332 nucleotide overlap with T. tyrosinosolvens and the species identification was confirmed by using the bioinformatics bacterial identification tool BIBI  (strain Bx-62986 closely related to type strain T. tyrosinosolvens DSM 44234). Second, this species identification based on the 16S rRNA gene was confirmed with another identification based on heat shock protein coding gene sequencing. The available hsp sequences of T. tyrosinosolvens strain IMMIB D-1411 and Tsukamurella paurometabola strain 2061 (GenBank accession numbers: TTU90204 and AF352578), respectively were aligned using multiple sequence alignment with hierarchical clustering  http://bioinfo.genotoul.fr/multalin/multalin.html webcite and analyzed to identify conserved regions for the primer design. The resulting primer set F2-HSP-Tsu (5′-AATTGCGTTCGACRAARAGG-3′) and R2-HSP-Tsu (5′-AATCCATGCCACCCATGC-3′) were designed using the web Primer3 software  http://frodo.wi.mit.edu/ webcite. Amplification parameters consisted of 95°C for 5 min, followed by 50 cycles of 95°C for 30 s and 60°C for 30 s, and 72°C for 90 s, and finally one cycle at 72°C for 5 min. Amplified DNA fragments were purified and direct sequencing was carried out on both strands (see above) with the PCR primers and the newly designed internal primers F3-HSP-Tsu (5′-GGACATGGCGATCCTCAC-3′) and R3-HSP-Tsu (5′-ACGATGGTGGTCTCGTCCT-3′). When compared with the NCBI Blast program, the 1576 nucleotide sequence had 96% identity in a 1579 nucleotide overlap while the deduced protein sequence had a 97% identity in a 524 residue overlap, thus confirming the 16S rRNA gene identification.
The 16S rDNA and hsp65 sequences from the T. tyrosinosolvens Bx-62986 strain were submitted to GenBank and assigned accession numbers FJ643548 and FJ643549, respectively.
A phylogenetic analysis was conducted on the nucleotide sequence of 16S rRNA gene, as well as on both the nucleotide and deduced amino acid sequences of the heat shock protein (HSP) from the isolate Bx-62986 and from other bacterial strains. Phylogenetic and molecular evolutionary analyses were conducted using the MEGA (Molecular Evolutionary Genetics Analysis) software version 4 . Nucleotide sequences of the 16S rRNA gene as well as nucleotide and amino acid sequences of the HSP were downloaded from GenBank databases (except for the Bx-62986 isolate) and aligned using the multiple alignment options in CLUSTAL followed by minor manual adjustments. Phylogenetic trees were generated by the neighbor-joining method. Branching significance was estimated using bootstrap confidence levels by randomly resampling the data 1000 times with the referred evolutionary distance model. Both trees were similar. With regard to HSP sequences, higher bootstrap values were obtained with protein sequences than with nucleotide sequences (data not shown). Both trees were highly similar and the analysis placed most sequences into species-specific clusters (Figure 1). As expected, the isolate Bx-62986 was closely related to T. tyrosinosolvens. Globally, the high bootstrap values obtained with the HSP protein sequences suggest that this protein could be useful for phylogenetic analyses.
Figure 1. Phylogenetic analysis of 16S rRNA and HSP generated with the neighbor-joining method. The phylogeny presented is based on the alignment of 1341 bp of the 16S rRNA and 201 residues of the HSP sequences. Theses consensus trees were rooted on the sequences of Rhodococcus equi, Nocardia asteroides, Mycobacterium abscessus and Dietzia maris (except for the HSP sequence not available for this species). Numbers next to each node indicate bootstrap values as a percentage of 1000 replicates. The bootstrap values below 50% are indicated by a slash (/). Each strain designation is followed by the corresponding Genbank accession number in parentheses. Asterisks indicate a Genbank accession numbers corresponding to a nucleotide sequence whose amino acid sequence was deduced.
Human infections with T. tyrosinosolvens are very rare with only six cases reported in the literature. In all of the cases, this bacterium was isolated in immunocompromized patients with gastric cancer  or chronic pulmonary infections  and in patients who were carriers of cardiac implants or intravascular catheters [15-17].
Tsukamurella species are strictly aerobic Gram-positive rods which can be easily misidentified as Corynebacterium species, Nocardia species or Mycobacteria species. The difficulties encountered in the identification of these bacteria were previously reported by Almuzara et al  who noted that the identification by the API Coryne bacterium System is limited and by Stanley et al  who failed in identifying them with GenoType. The difficulty in identifying the organism by phenotypic methods suggests that T. tyrosinosolvens and related species may well be underdiagnosed as a cause of pulmonary infections. In our case, blood culture helped to determine the etiology of pulmonary disease.
Sequence-based identification is an alternative method to identify clinical isolates that are either slow growers or difficult to identify by biochemical profiling. The 16S rRNA gene is the most frequent target used in clinical laboratories for molecular identification, but in some cases this tool can only help to identify the genus and it is not discriminative enough for speciation within the genus. Under such circumstances, sequences of essential genes such as hsp have been shown to be useful for the identification.
This report finally highlights the difficulty in identifying the genus Tsukamurella. There is currently no commercially available test for Gram-positive rods for the identification of Tsukamurella spp. Genotypic methods are therefore the logical solution to identify certain bacterial isolates. Unfortunately, they have not yet become routine methods in the laboratory. Nevertheless, they may be useful for epidemiological characterization of unusual causal agents of bacteremia in immunocompromized patients.
Written informed consent was obtained from the patient for publication of this case report.
The authors declare that they have no competing interests.
AM and SD carried out the experimental design of the study. AM performed genotypic identifications and phylogenetic analyses. SD, OP and JM carried out bacterial cultures and phenotypic identifications. AM, TDTN, CD and JM co-drafted the manuscript. All authors read and approved the final manuscript.
The authors are grateful to Elodie Sifré for technical assistance and to Francis Mégraud for manuscript review.
1.Chun J, Goodfellow M: A phylogenetic analysis of the genus Nocardia with 16S rRNA gene sequences.
International journal of systematic bacteriology 1995, 45(2):240-5.
2.Nam SW, Chun J, Kim S, Kim W, Zakrzewska-Czerwinska J, Goodfellow M: Tsukamurella spumae sp. nov., a novel actinomycete associated with foaming in activated sludge plants.
Systematic and applied microbiology 2003, 26(3):367-75. Publisher Full Text
3.Tsukamura M, Mizuno S: [A new species Gordona aurantiaca occurring in sputa of patients with pulmonary disease].
Kekkaku 1971, 46(4):93-8. PubMed Abstract
4.Alcaide ML, Espinoza L, Abbo L: Cavitary pneumonia secondary to Tsukamurella in an AIDS patient. First case and a review of the literature.
The Journal of infection 2004, 49(1):17-9.
5.Rey D, Fraisse P, Riegel P, Piemont Y, Lang JM: [Tsukamurella infections. Review of the literature apropos of a case].Pathologie-biologie 1997, 45(1):60-5. PubMed Abstract
6.Yassin AF, Rainey FA, Burghardt J, et al.: Tsukamurella tyrosinosolvens sp. nov.
International journal of systematic bacteriology 1997, 47(3):607-14.
7.Suzuki MT, Giovannoni SJ: Bias caused by template annealing in the amplification of mixtures of 16S rRNA genes by PCR.
Applied and environmental microbiology 1996, 62(2):625-30.
8.Park NY, Chung CY, McLaren AJ, Atyeo RF, Hampson DJ: Polymerase chain reaction for identification of human and porcine spirochaetes recovered from cases of intestinal spirochaetosis.
FEMS microbiology letters 1995, 125(2-3):225-9.
9.Altschul SF, Madden TL, Schaffer AA, et al.: Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.
Nucleic Acids Res 1997, 25(17):3389-402.
10.Devulder G, Perriere G, Baty F, Flandrois JP: BIBI, a bioinformatics bacterial identification tool.
Journal of clinical microbiology 2003, 41(4):1785-7.
11.Corpet F: Multiple sequence alignment with hierarchical clustering.
Nucleic Acids Research 1988, 16(22):10881-90.
12.Rozen S, Skaletsky H: Primer3 on the WWW for general users and for biologist programmers.
Methods in molecular biology (Clifton, NJ) 2000, 132:365-86.
13.Tamura K, Dudley J, Nei M, Kumar S: MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0.
Molecular biology and evolution 2007, 24(8):1596-9.
14.Matsumoto T, Shiraishi M, Yoshimura H, et al.: [Tsukamurella tyrosinosolvens cultured from sputum of a patient who received total gastrectomy for gastric cancer].
Kekkaku 2006, 81(7):487-90. PubMed Abstract
15.Elshibly S, Doherty J, Xu J, et al.: Central line-related bacteraemia due to Tsukamurella tyrosinosolvens in a haematology patient.
The Ulster medical journal 2005, 74(1):43-6.
16.Bouza E, Pérez-Parra A, Rosal M, Martín-Rabadán P, Rodríguez-Créixems M, Marín M: Tsukamurella : a cause of catheter-related bloodstream infections.
Eur J Clin Microbiol Infect Dis 2009, 28(2):203-10.
17.Sheridan EA, Warwick S, Chan A, Dall’Antonia M, Koliou M, Sefton A: Tsukamurella tyrosinosolvens intravascular catheter infection identified using 16S ribosomal DNA sequencing.
Clin Infect Dis 2003, 36(5):e69-70.
18.Almuzara MN, De Mier C, Rodriguez CR, Famiglietti AM, Vay CA: [Evaluation of API Coryne System, version 2.0, for diphteroid gram-positive rods identification with clinical relevance].
Revista Argentina de microbiologia 2006, 38(4):197-201.
19.Stanley T, Crothers L, McCalmont M, et al.: The potential misidentification of Tsukamurella pulmonis as an atypical Mycobacterium species: a cautionary tale.
Journal of medical microbiology 2006, 55(Pt 4):475-8.